Journal of Research in Pharmacy Practice

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 3  |  Issue : 1  |  Page : 19--22

Stability of antibacterial activity of Chlorhexidine and Doxycycline in bovine root dentine


Abbas Ali Khademi1, Marjan Saleh2, Masoud Khabiri2, Sanaz Jahadi3 
1 Department of Endodontics, Torabinejad Dental Research Center, Iranian Academy of Medical Science, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Endodontics, Islamic Azad University of Khorasgan, Isfahan, Iran
3 Department of Endodontics, Dental Implant Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Correspondence Address:
Sanaz Jahadi
Department of Endodontics, Dental Implant Research Center, Isfahan University of Medical Sciences, Isfahan
Iran

Objective: The aim of this study was to compare the substantivity of chlorhexidine (CHX) and doxycycline bond to the dentin in diffusion disk method. Methods: A total of 92 dentin disks were prepared from Bovine«SQ»s teeth root. After removing cementum and standardizing disks in weight and outer diameter, they were irrigated with sodium hypochlorite 2.5%, ethylenediaminetetraacetic acid 17% and sterile saline and sterilled in autoclave. Then, the first group (n = 36) were irrigated with chlrohexine 2% and the second group (n = 36) with doxycycline 100 mg/mL, each for a period of 10 min. The third group (n = 20) was considered as the control group. Samples were divided into four subgroups, which after 1 day, 3, 6, and 12 weeks were incubated in plates containing Muller Hinton agar broth and Enterococcus faecalis for a period of 48 h at 37°C temperature; then the diameter of the zone of inhibition was measured. The antimicrobial effect of irrigating solutions without binding to the dentin was also studied using the plate well method. One-way and univariate variance tests as well as Post-hoc Tukey were used for data analyses. Findings: The diameter of the zone of inhibition of doxycycline group was significantly more than the CHX group from the beginning to the 3 rd week (P < 0.005). After 3 rd week, mean diameter in doxycycline group declined as compared with the CHX group up to 12 th week (P < 0.005). The difference between means in two groups was not significant in the 3 rd week (P = 0.87). Conclusion: The results of this study show that CHX and doxycycline show antibacterial activity for 12 weeks; although after 3 rd week, the substantivity of CHX was significantly greater than doxycycline. Both of these irrigants can be used in other pharmacological and medical fields whenever a long-lasting antibacterial action is needed.


How to cite this article:
Khademi AA, Saleh M, Khabiri M, Jahadi S. Stability of antibacterial activity of Chlorhexidine and Doxycycline in bovine root dentine.J Res Pharm Pract 2014;3:19-22


How to cite this URL:
Khademi AA, Saleh M, Khabiri M, Jahadi S. Stability of antibacterial activity of Chlorhexidine and Doxycycline in bovine root dentine. J Res Pharm Pract [serial online] 2014 [cited 2020 Jul 12 ];3:19-22
Available from: http://www.jrpp.net/article.asp?issn=2319-9644;year=2014;volume=3;issue=1;spage=19;epage=22;aulast=Khademi;type=0